Human Transglutaminase 2/TGM2 PharmaGenie ELISA Kit (SBRS1145)

Built for research into tissue transglutaminase biology and its roles in celiac disease, fibrosis, and oncology, this 96-assay ELISA kit from Assay Genie enables quantitative measurement of human TGM2 protein. The kit supplies reagents in a microplate format and produces a colorimetric readout compatible with standard plate-reader workflows, supporting medium-throughput screening programs where altered TGM2 expression or release is a biomarker of interest.

The immunoassay uses paired antibodies specific to human TGM2, with the capture antibody immobilized on the plate. Researchers add standards and samples, followed by a biotinylated detection antibody and streptavidin-HRP conjugate. After substrate addition, the enzymatic reaction generates a measurable color signal proportional to TGM2 concentration, which is interpolated from a standard curve. The protocol accommodates cell culture supernatant as well as plasma and serum specimens from human donors, with recommended dilutions provided in the kit insert.

Typical applications include profiling TGM2 secretion from cultured cells, monitoring circulating TGM2 levels in clinical cohorts, and supporting drug-discovery screens that target transglutaminase activity in fibrotic or tumor microenvironments. The kit is intended for research use only, and components should be stored according to the package insert to preserve antibody binding activity and standard stability across runs.