Human Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

The Assay Genie Human Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit enables quantitative measurement of TGF-β3 in human samples such as serum, plasma, tissue homogenates, and cell culture supernatants using a microplate-based immunoassay with a capture antibody specific to the human cytokine. TGF-β3 is a 25 kDa homodimeric cytokine belonging to the TGF-β superfamily and plays central roles in scarless wound healing, palatogenesis, cardiac valve morphogenesis, and immune regulation, supporting its application across regenerative medicine, developmental biology, oncology, and fibrosis research. The assay uses 100 µL of sample per well, with standards and unknowns typically run in duplicate for accurate interpolation from the standard curve. Because circulating TGF-β3 is often sequestered within a latent complex with latency-associated peptide, an activation step is generally performed before the assay to release free cytokine for accurate quantification. Reagents should be stored as indicated in the kit insert, with the reconstituted standard aliquoted to limit freeze-thaw cycles and preserve antigen binding activity. This format supports medium-throughput studies of patient cohorts, treatment response, or developmental time courses where TGF-β3 expression changes are hypothesized to drive the experimental phenotype.