Human Transferrin Receptor (TFR) ELISA Kit

Quantitative measurement of human transferrin receptor (CD71/TfR1) protein is supported by this ELISA kit from Assay Genie, intended for research use in iron metabolism studies, anemia research, and investigations of malignant cell proliferation. The colorimetric assay uses standard microplate reader absorbance detection, and sample preparation can begin with cell lysates from cultured cell lines, tissue homogenates, or matched serum fractions and plasma fractions from human donors, with each kit providing reagents sufficient for 96 individual determinations.

Transferrin receptor 1 mediates cellular iron uptake via clathrin-mediated endocytosis of diferric transferrin, making the receptor a key target in studies of iron homeostasis, erythropoiesis, and rapid proliferation in tumor tissue. Soluble transferrin receptor (sTfR) levels in circulation also serve as an indicator of erythropoietic activity and iron status, supporting research on iron-deficiency anemia, anemia of chronic disease, and disorders of iron overload. Antibody-drug conjugate developers likewise evaluate TfR1 expression because of its surface density on highly proliferative cells.

The kit ships with pre-coated plates, detection antibodies, calibrated standards, and substrate reagents needed to generate standard curves. Store the kit at 4 degrees C upon arrival to preserve antibody binding activity, and protect reagents from light. The kit is intended for in vitro research use only and is not approved for diagnostic procedures. Each package is supplied in a microplate format with reagents sufficient for 96 determinations.