Human Transcriptional repressor CTCFL (CTCFL) ELISA Kit

An immunoassay from Assay Genie designed for the quantitative detection of human Transcriptional repressor CTCFL (CTCFL), also referred to by synonyms including BORIS, CCCTC-binding factor, CTCF paralog, CTCF-like protein, CT27, and Zinc finger protein CTCF-T [C0, S1]. The kit is configured as a sandwich ELISA [C0] and is validated for use with human serum and plasma samples [C0], with additional tested matrices including tissue homogenates, cell culture supernatants, and other biological fluids [S1].

CTCFL is a testis-specific DNA binding protein involved in insulator function, nuclear architecture, transcriptional control, and epigenetic chromatin modification, playing a key role in gene imprinting in the male germline [S1]. Dysregulation of CTCFL has been associated with cancer and developmental disorders, supporting its value as a research biomarker [S1]. According to manufacturer specifications, the assay has a detection range of 0.312-20 ng/mL and a sensitivity of 0.19 ng/mL [S1]. Reported precision values are an intra-assay CV of 6.3% and an inter-assay CV of 11.5% [S1]. Linearity was demonstrated in serum, EDTA plasma, and heparin plasma at dilutions from 1:2 to 1:16, with recovery averaging 97% in serum (range 91-103) and 99% in plasma (range 93-105) [S1].

The kit is specific for natural and recombinant human Transcriptional repressor CTCFL and recognises the protein mapped to UniProt entry Q8NI51 [S1]. Subcellular localisation is reported as cytoplasm and nucleus, and the listed research area is epigenetics [S1]. The product is labelled for research use only [S1].