Human Transcription factor HES-1 (HES1) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the precise quantification of Human Transcription factor HES-1 (HES1) levels in human research samples. The assay targets the bHLH-family transcriptional repressor HES1, which regulates cellular processes such as cell differentiation and proliferation and has been linked to cancer, developmental disorders, and neurodegenerative conditions. The kit is intended for research use only and supports work in the Epigenetics research area.

Quantitative performance figures quoted from the product specification include a detection range of 78-5000pg/mL, a sensitivity of 39.7pg/mL, an intra-assay CV of 6.7%, and an inter-assay CV of 10.3%. Linearity has been verified in serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16, with reported recovery averages of 108% for serum and 110% for plasma.

The kit accepts serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, and shows specificity for natural and recombinant human Transcription factor HES-1. HES1 localizes to the nucleus (UniProt Q14469) and functions as a transcriptional repressor of genes requiring a bHLH protein for transcription, binding N-box motifs (5'-CACNAG-3') with high affinity and E-box motifs (5'-CANNTG-3') with low affinity.