Human TRAIP ELISA Kit (HUFI05991)

The Assay Genie Human TRAIP ELISA Kit quantifies TRAIP (TRAF-Interacting Protein), an E3 ubiquitin ligase essential for DNA replication fork protection, homologous recombination repair, and mitotic checkpoint control. Biallelic loss-of-function mutations cause primordial dwarfism with severe microcephaly, while upregulated TRAIP expression supports cancer cell survival under replication stress and genotoxic pressure. Researchers in DNA damage response, neurodevelopment, and tumor biology use this kit to track endogenous TRAIP abundance across patient cohorts and cultured cell models.

Built around a human-specific capture antibody raised against recombinant TRAIP, the workflow pairs a biotin-conjugated detection reagent with streptavidin-HRP readout on a microplate with separable strip wells, sufficient for 96 individual determinations. The standard curve includes a serial dilution series of recombinant TRAIP calibrator, and the protocol accommodates approximately 100 µL of sample per well. Kit components arrive refrigerated; reconstituted standards should be aliquoted and frozen to maintain activity across repeated runs, and the sealed plate should be kept dry and protected from light. Intended for research use only — not for diagnostic or therapeutic procedures.