Human TRAIL R2 PharmaGenie ELISA Kit (SBRS1182)

The TRAIL R2 receptor, also called DR5 or TNFRSF10B, is a cell-surface death receptor that triggers pro-apoptotic signaling when engaged by TNF-related apoptosis-inducing ligand. Research teams working on cancer cell apoptosis, immune regulation, and death-receptor pharmacology use ELISA-based quantification to track TRAIL R2 expression and shedding in their experimental systems. The Assay Genie Human TRAIL R2 PharmaGenie ELISA Kit provides a quantitative immunoassay workflow for measuring this receptor across cell culture supernatants and human sample matrices.

Configured for 96 assays per plate, the kit uses a colorimetric readout read on a standard microplate reader. Each kit contains pre-coated microplate strips, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, assay diluent, standard diluent, recombinant TRAIL R2 standard, and stop solution supplied in individually labeled vials. The capture antibodies specifically recognize human TRAIL R2, allowing selective measurement in human-derived material with low cross-reactivity toward the related death receptor TRAIL R1, also known as DR4.

The protocol involves sequential incubation and wash steps, with a standard curve generated from serial dilutions of the recombinant reference standard used to interpolate unknown sample concentrations. All reagents are supplied for research use only, and components should be handled according to standard laboratory practices. This kit supports apoptosis research, oncology biomarker studies, and investigations into TRAIL-pathway therapeutics.