Human TPCN1 ELISA Kit (HUFI08356)

TPCN1 (Two-Pore Channel 1) is an endolysosomal ion channel that mediates calcium release from acidic organelles and contributes to NAADP-evoked signaling, autophagy, and vesicular trafficking. The Human TPCN1 ELISA Kit from Assay Genie provides a quantitative immunoassay for native TPCN1 protein, supporting research into lysosomal calcium handling, ion channel biology, and the cellular mechanisms implicated in metabolic and neurodegenerative disease models. Researchers using this kit commonly work with human-derived material and read results on a standard microplate reader, then validate findings with PCR, western blot, or flow cytometry workflows.

The kit is supplied as a complete reagent set with pre-coated plate, detection antibody, calibrated standards, wash buffer, substrate, and stop solution. Components should be stored refrigerated and protected from light; reconstituted standards should be aliquoted to limit freeze-thaw cycles, and the substrate must be kept away from direct illumination during the assay. Each kit supports ninety-six determinations in a single-use microplate layout, intended for research use only. Typical applications include profiling TPCN1 expression in cell culture lysates, comparing channel abundance across disease and control cohorts, and following channel modulation after pharmacological treatment of cultured cells.