Human TP53BP2 (Apoptosis-stimulating of p53 protein 2) ELISA KIT (HUFI04813)

The Human TP53BP2 ELISA Kit from Assay Genie enables quantitative measurement of ASPP2 (Apoptosis-Stimulating Protein of p53, 2), a ~125 kDa tumor suppressor scaffold that potentiates p53-dependent apoptosis and modulates DNA damage response signaling. The kit supports cancer biology research, studies of apoptotic regulation, and investigations into p53 pathway activity in clinical and experimental specimens.

Each kit is supplied with a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP reagents, calibrated protein standards, wash buffer, and substrate solution. Standard storage temperature is 4°C; reagents should be protected from light and the kit's shelf life observed. The recommended sample volume is 100 µL per well. Detection is based on a biotin-streptavidin amplification system with TMB substrate, and the capture antibody is human-specific, with no significant cross-reactivity observed with mouse or rat ASPP2 homologs under standard conditions.

Common research applications include profiling ASPP2 expression in tumor biopsies, monitoring TP53BP2 levels in cancer cell lines following drug treatment, and evaluating apoptosis regulators in translational studies. This ELISA is intended for research use only and supports in vitro investigations of tumor suppressor function, cell cycle checkpoint activity, and DNA damage response pathways.