Human TOP1MT ELISA Kit (HUFI06013)

Mitochondrial topoisomerase I (TOP1MT) is the only mammalian topoisomerase that localizes to mitochondria, where it relieves torsional stress in mitochondrial DNA during replication and transcription. This Assay Genie ELISA kit enables researchers to quantify TOP1MT protein levels in human biological specimens, supporting studies of mitochondrial genome maintenance, oxidative phosphorylation regulation, and the role of mitochondrial topoisomerase activity in cancer and metabolic disease research.

The kit is configured as a complete immunoassay with all reagents required to generate a standard curve and run samples. Researchers commonly apply this ELISA to in vitro cell culture experiments and primary tissue lysates to investigate TOP1MT expression patterns across tissues with high mitochondrial content. The assay format supports studies of mitochondrial dysfunction in neurodegeneration, myopathies, and oncology.

Intended for research use only, the kit provides a quantitative tool for laboratories exploring mitochondrial DNA topology, mtDNA replication mechanisms, and TOP1MT's emerging role in tumor metabolism and chemotherapy response.