Human TOMM34 ELISA Kit (HUFI06010)

The TOMM34 protein (Translocase of Outer Mitochondrial Membrane 34, approximately 34 kDa) functions as a mitochondrial chaperone that facilitates the import of precursor proteins into mitochondria. Research has linked elevated TOMM34 expression to hepatocellular carcinoma and colorectal cancer, with additional investigation in lung and breast cancer contexts, making it a target of interest in oncology biomarker studies.

This Assay Genie kit is configured as a quantitative immunoassay. The plate is pre-coated with human-specific capture antibody that selectively binds TOMM34 without significant cross-reactivity with related outer membrane translocases such as TOMM20 or TOMM40. Researchers load 100 µL of prepared sample per well, incubate, wash, and add detection reagents.

Standard curve preparation involves serial dilution of the provided recombinant reference standard to generate a quantitative range spanning low to mid ng/mL concentrations. Assay components include pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer, and standard diluent.

Storage guidance recommends keeping the kit refrigerated between uses, with reconstituted standards and detection antibody stored frozen in single-use aliquots to limit freeze-thaw degradation. Reconstituted streptavidin conjugate should be protected from light. All reagents are designated for research use only.