Human Toll/interleukin-1 receptor domain-containing adapter protein (TIRAP) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the precise measurement of human Toll/interleukin-1 receptor domain-containing adapter protein (TIRAP), also referred to as Adaptor protein Wyatt, MyD88 adapter-like protein, MyD88-2, TIR domain-containing adapter protein, or MAL. The kit is reactive with human samples and validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, providing a reliable tool for immunology research into Toll-like receptor signaling, inflammatory processes, infectious diseases and autoimmune disorders.

TIRAP is an adaptor protein involved in TLR2 and TLR4 signaling pathways in the innate immune response. It acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. The protein positively regulates the production of TNF-alpha and interleukin-6. The assay shows specificity for natural and recombinant human TIRAP (UniProt P58753) and is intended for research use only.

Quantitative performance specifications include a detection range of 31.2-2000pg/mL, a sensitivity of 15pg/mL, an intra-assay CV of 4.6% and an inter-assay CV of 8.3%. Reported recovery is 95% (range 89-101) for serum and 97% (range 91-103) for plasma.