Human TNK1 ELISA Kit (HUFI06026)

Quantitative measurement of tyrosine kinase non-receptor type 1 (TNK1) protein in human research specimens is delivered by this Assay Genie ELISA Kit, supporting investigations of kinase signaling cascades linked to hematopoietic malignancies and solid tumor proliferation. TNK1 was first characterized as a Ras-activated non-receptor tyrosine kinase, and altered TNK1 expression has since been reported in chronic lymphocytic leukemia, diffuse large B-cell lymphoma, and related oncogenic signaling programs, making reproducible quantification valuable for translational research programs.

Supplied as a complete 96-reaction kit, the reagents include a pre-coated microplate, lyophilized TNK1 calibrator standards, biotinylated detection reagent, streptavidin conjugate, wash concentrate, substrate, and stop solution. Reconstituted components should be aliquoted, frozen at -20°C, and protected from light to preserve activity through repeated freeze-thaw cycles, while the unopened kit remains refrigerated at 4°C until first use. All reagents are calibrated for in vitro laboratory work.

Validated human specimen matrices include serum samples and plasma fractions collected by standard venipuncture, with the assay using a low microliter sample volume per well. Applications span baseline expression profiling of TNK1 in cell culture lysates, pathway studies in primary tissue samples, and treatment-response monitoring across hematological and solid tumor models, all supported by the qualitative and quantitative end-point readout supplied in this 96-assay kit from Assay Genie.