Human TNIP2 (TNFAIP3 interacting protein 2) ELISA Kit (HUFI06025)

The Assay Genie TNIP2 ELISA Kit provides researchers with a quantitative tool to measure TNFAIP3 interacting protein 2 (TNIP2), also known as ABIN-2, a negative regulator of NF-kB signaling that interacts with the deubiquitinase A20/TNFAIP3. TNIP2 modulates inflammatory cascades and has been implicated in autoimmune disease mechanisms, innate immunity, and chronic inflammatory signaling research.

The kit format includes a pre-coated microplate, biotinylated detection antibody, recombinant protein standards, and assay buffers supplied in vials, supporting 96 individual determinations per plate. Allow all reagents to equilibrate to room temperature before use, and store the kit refrigerated between runs. For longer-term stability, the reconstituted standard should be kept frozen at -20 degrees C and protected from light. Aliquot the standard upon first reconstitution to avoid repeated freeze-thaw cycles, which can degrade recombinant protein integrity.

This in vitro assay is intended for research use only and supports investigations into NF-kB pathway dysregulation, A20-binding protein interactions, and biomarker discovery in chronic inflammatory conditions such as rheumatoid arthritis, inflammatory bowel disease, and systemic lupus erythematosus. The assay is validated for human TNIP2 detection with species-specific reactivity in serum or plasma specimens, enabling translational studies linking TNIP2 expression to disease severity and treatment response.