Human TNF-beta PharmaGenie ELISA Kit (SBRS1167)

For research profiling lymphotoxin-alpha (TNF-beta/LT-α) activity in human immune studies, this colorimetric ELISA kit from Assay Genie enables quantitative measurement of TNF-beta across cell culture supernatant, plasma samples, and serum samples. TNF-beta (also known as LT-α) is a pro-inflammatory cytokine secreted primarily by activated lymphocytes, with established roles in secondary lymphoid organ development, germinal center formation, and coordination of cell-mediated immune responses. The kit supports immunology research into inflammatory disease mechanisms, autoimmune pathways, host-pathogen interactions, and tumor microenvironment signaling where TNF-beta activity through TNFR1, TNFR2, and LT-βR receptors drives pathology.

The 96-assay microplate workflow includes pre-coated plates, detection antibody, recombinant standards, and substrate reagents required to build a standard curve for interpolating unknown sample concentrations. Researchers studying cytokine networks in disease models can apply this kit to compare TNF-beta levels across treatment conditions, patient cohorts, or modified cell lines. Colorimetric readout enables straightforward quantification on a standard microplate absorbance reader.

For in vitro research use, the assay accepts diverse sample matrices, including cell culture supernatant from stimulated PBMC cultures, human plasma, and human serum. Each kit ships with sufficient reagents for the stated number of determinations and should be stored refrigerated to preserve antibody and standard integrity. Standard ELISA workflow applies, with wash, incubation, and detection steps performed according to the supplied protocol.