Human TMEM35 ELISA Kit (HUFI06052)

This TMEM35 quantitative immunoassay from Assay Genie enables researchers to measure transmembrane protein 35 levels in human biospecimens, supporting studies of membrane protein expression, cellular transport biology, and biomarker discovery. TMEM35, a multi-pass transmembrane protein of largely uncharacterized function, has gained attention in transcriptomic surveys and gene expression databases, making targeted protein-level validation an important confirmatory step in biomedical workflows.

The kit is configured in a 96 Assays microplate format and is supplied with pre-coated capture wells, biotinylated detection antibody, streptavidin-HRP conjugate, calibrator standards, wash buffer, and chromogenic TMB substrate. Each well is loaded with 100 µL of sample, with unknowns assayed against a calibrator standard curve and absorbance read on a standard microplate reader. The protocol follows standard immunoassay steps with recommended dilutions and incubation times detailed in the provided manual.

For storage, keep the sealed kit refrigerated and protect the substrate vial and conjugate from light; avoid repeated freeze-thaw of antibody aliquots. Allow all components to reach room temperature before running the assay. The kit is supplied with sufficient reagents for 96 determinations in the listed format and is intended for research use only, not for clinical diagnostics.