Human TMEM154 ELISA Kit (HUFI06066)

TMEM154 (Transmembrane Protein 154) is a multi-pass integral membrane protein residing in the endoplasmic reticulum, with single nucleotide variants studied in the context of lentiviral susceptibility. Investigators examining viral cofactors, membrane protein trafficking, or comparative immunogenetics use this ELISA to quantify TMEM154 levels in clinical liquid specimens.

Assay Genie supplies the kit as a microplate package with pre-coated capture antibody, lyophilized recombinant standard, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and assay diluents. Store unopened components at 4 degrees C; protected from light, the reagents remain stable through the printed expiration date. Bring all reagents to room temperature (18 to 25 degrees C) before use, and avoid more than three freeze-thaw cycles of reconstituted standards.

Each microplate contains 12 strips of 8 break-apart wells, supporting duplicate measurements and a standard curve generated from serial dilutions of the recombinant standard. No significant cross-reactivity has been observed with homologous transmembrane family members during assay validation. The package insert details the protocol, dilution recommendations, and analysis guidelines for accurate quantification.