Human TLE1 ELISA Kit (HUFI06088)

This ELISA kit from Assay Genie measures TLE1 protein in human research samples. TLE1 (Transducin-Like Enhancer of Split 1) is a transcriptional corepressor of the Groucho family that downregulates Notch and Wnt pathway output, and is studied in synovial sarcoma pathology, hematopoietic lineage commitment, and neurodevelopmental contexts. The kit ships as a complete configuration sufficient for 96 individual determinations, with components packaged in vials containing capture antibody, pre-coated plates, lyophilized recombinant TLE1 standards, biotinylated detection reagent, streptavidin conjugate, substrate solution, stop reagent, and concentrated wash buffer. Reconstituted standards and diluted samples are loaded into wells, the colorimetric read taken at the appropriate absorbance wavelength, and concentrations interpolated from the standard curve. Stable storage is maintained refrigerated until the printed expiration date on the outer box. Each kit arrives ready for quantitative in vitro analysis of soluble TLE1, supporting oncology, signal transduction, and developmental biology laboratories that need a reproducible immunoassay readout. The format enables high-throughput cohort studies, with researchers commonly processing sample sets alongside an internal control included in the pack to confirm plate-to-plate reproducibility. Application areas include screening TLE1 overexpression in patient-derived sarcoma specimens, comparing expression levels across differentiated versus undifferentiated hematopoietic progenitors, and characterizing pathway activity in cell culture models where the corepressor function is under investigation.