Human Tissue factor/Coagulation Factor III ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit enables accurate and precise detection of tissue factor (coagulation factor III) levels in human samples. Tissue factor is a key initiator of the blood coagulation cascade, and its dysregulation has been implicated in pathological conditions such as thrombosis, inflammation, and cancer, making it valuable for a broad range of research applications.

The assay is configured for human reactivity and uses a double-antibody sandwich ELISA format with colorimetric detection. It delivers a sensitivity of 4.688 pg/mL and a working range of 7.813–500 pg/mL. Acceptable sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the kit components are stored at 2–8°C.

The workflow involves equilibrating reagents and TMB substrate to room temperature, loading standards and samples onto the pre-coated plate for a 90-minute primary incubation at 37°C, binding with a biotin-labelled detection antibody, adding HRP-Streptavidin, developing colour with TMB substrate in the dark for 10–20 minutes, and reading absorbance at 450 nm immediately after adding the stop solution.