Human TINAG ELISA Kit (HUFI06094)

TINAG (Tubulointerstitial Nephritis Antigen-Like) is a glycoprotein first characterized in renal tubular basement membranes, where it contributes to extracellular matrix organization and cell-matrix adhesion in the kidney. The Assay Genie ELISA kit supports quantitative measurement of this antigen in research settings and is supplied in a 96 Assays kit format.

This in vitro immunoassay is intended for research use only and serves investigators working on tubulointerstitial pathophysiology, renal biomarker discovery, and matrix biology. Typical applications include profiling TINAG expression in cell culture models of tubular injury, examining changes during kidney development, and screening experimental cohorts for altered tubular basement membrane turnover. The assay fits into broader studies of nephritis-related antigens, glomerular versus tubular disease markers, and adhesion molecule biology.

Standard ELISA workflow applies, using pre-coated plate wells, sequential incubation with standards and samples, biotinylated detection antibody, streptavidin-HRP conjugate, substrate development, and absorbance readout on a plate reader. Researchers should refer to the enclosed protocol for recommended sample dilution, incubation times, and curve-fitting parameters specific to their experimental system. Proper storage of reagents and protection of the substrate from light are required to preserve assay performance across the full plate.