Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit (HUES02489)

Manufactured by Assay Genie, this Sandwich-ELISA kit is intended for the quantitative measurement of human Tissue Inhibitor of Metalloproteinase 3 (TIMP-3) in serum, plasma and other biological fluids using a colorimetric detection method. The protocol uses a 100 μL sample volume and is completed in 3 h 30 min.

Performance characteristics stated by the manufacturer include a sensitivity of 37.5 pg/mL, a detection range of 62.5–4000 pg/mL and a recovery of 80%–120%. The kit is reported to recognise human TIMP-3 with no significant cross-reactivity or interference from analogues. Linearity has been characterised in serum, EDTA plasma and cell culture media at dilutions from 1:2 to 1:16, and intra-assay and inter-assay precision data are provided, with CV values ranging from 4.31% to 7.37%.

Recommended for research in cancer, cell biology, cardiovascular biology, neuroscience and signal transduction, the kit is supplied with a technical manual and a certificate of analysis. Components are stored at 2–8°C or −20°C as listed in the kit insert.