Human Thyrotropin-releasing hormone PharmaGenie ELISA Kit (SBRS0175)

The Thyrotropin-releasing hormone (TRH) PharmaGenie kit from Assay Genie enables quantitative measurement of this tripeptide neurohormone, which is synthesized in the hypothalamus and acts as the principal regulator of the hypothalamic-pituitary-thyroid axis. TRH stimulates anterior pituitary release of thyrotropin (TSH) and prolactin, making this kit useful for studies of central neuroendocrine regulation, stress-related hormone responses, and pituitary feedback mechanisms. The competitive immunoassay format suits cell culture supernatant analysis from hypothalamic neurons or pituitary explants, as well as serum samples from clinical research cohorts investigating thyroid dysfunction, mood disorders, and circadian rhythm disturbances. Each well plate is configured for 96 assays, with all reagents provided ready-to-use following brief equilibration. The standard curve spans approximately 15.6–1000 pg/mL with a reported detection sensitivity near 5 pg/mL, covering the physiologically relevant range for circulating TRH, which is normally present at low picomolar concentrations. Researchers should aliquot and freeze serum samples promptly after collection to preserve the labile tripeptide, and limit freeze-thaw cycles. The kit is intended for research use only and supports in vitro investigation of TRH biology in neuroendocrinology, reproductive biology, and behavioral neuroscience laboratories.