Human Thymosin Beta 4/TMSB4 ELISA Kit

From Assay Genie, this ELISA Kit is designed for the accurate quantitative measurement of Thymosin Beta-4 (TMSB4) levels in human samples. It supports research into Thymosin Beta-4, a peptide involved in wound healing, tissue repair, cell migration and tissue regeneration, and serves as a biomarker for investigating its role in various health conditions and potential therapeutic applications.

Key performance characteristics include a sensitivity of 18.75 pg/mL and a working range of 31.25–2000 pg/mL. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with storage at 2–8°C for 12 months. Linearity has been demonstrated across serum (86–104%), EDTA plasma (82–100%) and heparin plasma (84–100%) at 1:2, 1:4 and 1:8 dilutions. Recovery performance averages 91% (serum), 97% (EDTA plasma) and 100% (heparin plasma).

The protocol involves equilibrating reagents and TMB substrate to room temperature, incubating the plate at 37°C for 90 minutes for antigen binding, adding biotin-labelled detection antibody (60 minutes), HRP-Streptavidin binding (30 minutes), TMB color development in the dark for 10–20 minutes, and reading absorbance at 450 nm after adding stop solution. Sample preparation protocols cover serum (centrifuge at 1000 × g for 20 minutes), plasma (1000 × g for 15 minutes at 2–8°C), tissue homogenate, cell culture supernatant and cell lysate.