Human Thymidylate Synthetase (TYMS) ELISA Kit

TYMS, the enzyme thymidylate synthase, catalyzes the reductive methylation of deoxyuridine monophosphate to deoxythymidine monophosphate, an essential step in de novo pyrimidine biosynthesis. Because TYMS is the molecular target of clinically important antimetabolite drugs such as 5-fluorouracil, quantitative measurement of TYMS protein levels is widely used in cancer pharmacology and chemotherapy-response studies.

The Assay Genie Human Thymidylate Synthetase (TYMS) ELISA Kit provides a quantitative immunoassay for measuring TYMS in human specimens. The 96-assay plate format uses an absorbance-based chromogenic readout measured on a standard microplate spectrophotometer. Validated specimen types include heparin or EDTA plasma, serum preparations, and tissue homogenates, with the protocol accommodating additional body fluids as well. Each plate is pre-coated with capture antibody and is supplied with calibrated standards, detection reagents, and buffer solutions needed to construct a standard curve.

Configured for research use only, the kit is specific to human TYMS reactivity and supports diverse laboratory workflows, including screening of patient-derived samples, validation of cell-line expression levels in vitro, and analysis of xenograft or other in vivo model systems. Its quantitative readout makes it appropriate for comparing TYMS expression across experimental groups, characterizing drug-induced enzyme modulation, and exploring TYMS as a candidate biomarker in translational oncology research.