Human Thymidine Kinase 1, Soluble (TK1) ELISA Kit

Thymidine Kinase 1 (TK1) is a cytosolic enzyme that catalyzes phosphorylation of thymidine to thymidine monophosphate, a rate-limiting step in the salvage pathway of DNA precursor synthesis. Because TK1 expression rises sharply during S-phase, its circulating concentration serves as a proliferation marker. From Assay Genie, this quantitative immunoassay provides researchers with a tool to measure soluble TK1 protein in human specimens, supporting oncology biomarker development, tumor cell proliferation studies, and longitudinal monitoring of disease activity.

The kit uses an absorbance-based readout and includes all reagents required to run the assay in a 96-test configuration. It is validated for use with human-derived specimens including serum preparations, plasma samples, and tissue homogenates. Reconstituted standards and working solutions should be kept refrigerated and protected from light; long-term storage of unopened components is recommended at freezer temperatures to preserve activity through multiple freeze-thaw cycles.

Typical applications include cancer research, screening assay development, evaluation of proliferative activity in solid tumors and hematological malignancies, and assessment of treatment response. The kit is intended for in vitro research use only and is not validated for diagnostic procedures. Each package contains a pre-coated microplate, detection antibody, calibration standards, and buffer reagents sufficient for the stated number of determinations.