Human Thromboxane Receptor (TP) ELISA Kit

From Assay Genie, this thromboxane receptor quantification kit is built around a paired-antibody capture format that uses a monoclonal capture antibody pre-coated on the microplate. Researchers studying the TBXA2R gene, which maps to chromosome 19p13.3 and encodes a 343-amino-acid GPCR with TPα and TPβ splice variants, can apply this kit to investigate receptor abundance in platelet-rich samples, vascular smooth muscle preparations, and inflamed tissue models. The workflow accommodates a 100 µL loading volume and accepts crude homogenates without requiring prior enrichment steps such as affinity pull-down. Compared to radioligand binding assays that measure only functional TXA2 occupancy, antibody-based quantification reports total receptor protein, including internalized and uncoupled populations, which is useful for tracking receptor desensitization following agonist exposure. Stability data indicate that reconstituted standards remain active through at least three freeze-thaw cycles when stored aliquoted below -20 °C, and the complete kit shelf life is 12 months from the date of manufacture when refrigerated at 4 °C. Compatible downstream readouts include parallel transcript analysis of TBXA2R mRNA and orthogonal confirmation by western blot. For research use only.