Human Thrombospondin Type I Domain Containing Protein 7A (THSD7A) ELISA Kit

THSD7A is a large transmembrane glycoprotein expressed on glomerular podocytes and vascular endothelium, and it has emerged as a key biomarker in autoimmune kidney disease. Roughly 8 to 18% of patients with primary membranous nephropathy who are seronegative for anti-PLA2R1 antibodies carry circulating IgG4 autoantibodies directed against the THSD7A ectodomain, making quantitative measurement of THSD7A in tissue and cell samples a valuable tool for podocyte-injury research. This Assay Genie kit is configured as a 96 assays plate-based immunoassay for in vitro research use only.

Compatible sample types include kidney tissue homogenates, podocyte cell lysates, and related biospecimens, which lets laboratories profile THSD7A expression in biopsy material, glomerular cell culture models of podocyte stress, or vascular endothelial preparations. Standard microplate reader instrumentation is used to record the colorimetric endpoint, and the single-use plate format supports batch processing of clinical research cohorts. Unopened components should be stored refrigerated and protected from light; allow all reagents to equilibrate to room temperature before running the assay. The kit is intended for research use only and is not validated for diagnostic procedures.