Human Thrombomodulin/BDCA-3 ELISA Kit

A high-quality Sandwich ELISA from Assay Genie designed for the precise measurement of Thrombomodulin (BDCA-3) in human samples. This kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, making it suitable for diverse research applications in vascular biology, coagulation, and inflammation studies.

The assay delivers a sensitivity of 37.5 pg/mL with a detection range of 62.5–4000 pg/mL, employing a colorimetric detection method based on TMB substrate with absorbance measured at 450 nm. Performance validation includes linearity testing across serum, EDTA plasma, and heparin plasma samples, along with recovery studies demonstrating reliable quantification. Kit components are stable when stored at 2–8°C for up to 12 months.

Thrombomodulin, also known as BDCA-3, is a key protein involved in blood coagulation and regulation, playing a critical role in maintaining vascular health and preventing thrombosis. Dysregulation of this biomarker has been linked to various cardiovascular diseases, sepsis, and other inflammatory conditions, supporting its significance in disease research and therapeutic development. The standard assay workflow includes reagent equilibration, primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-Streptavidin conjugation, and TMB color development prior to stop reaction and reading.