Human Thrombin ELISA Kit (HUFI04750)

Quantify thrombin with this colorimetric ELISA configured for blood-derived specimens. Thrombin (coagulation factor IIa) is the central effector protease of the coagulation cascade, cleaving fibrinogen to fibrin and activating platelets along with factors V, VIII, XI, and XIII, which makes accurate quantification valuable in studies of hemostasis, thrombosis, hemophilia, atherosclerosis, and wound healing. The kit provides 96 Assays on a microplate and is for research use only. Reagents arrive refrigerated and should be allowed to equilibrate to room temperature before the assay; standards and detection antibodies are provided in single-use vials to preserve calibration. Avoid repeated freeze-thaw cycles of prepared working standards and aliquot samples on receipt. The Assay Genie kit is reactive with human thrombin and uses a colorimetric substrate read at the wavelength specified in the product insert. Researchers should consult the lot-specific certificate of analysis for exact standard curve concentrations, recommended sample dilution ranges, and storage conditions.