Human THRA/Thyroid Hormone Receptor alpha ELISA Kit

Designed for the quantitative measurement of Thyroid Hormone Receptor Alpha in human samples, this Sandwich ELISA kit from Assay Genie uses a double-antibody, colorimetric detection workflow validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay is intended for use in Epigenetics and Nuclear Signaling research, and the kit can be stored at 2-8°C for 12 months.

The reported sensitivity is 0.938 mIU/mL, with a measurable range of 1.563-100 mIU/mL. Reported linearity for diluted samples spans 86-105% across serum, EDTA plasma, and heparin plasma matrices, and spike-and-recovery averages fall between 96% and 97% across the same matrices. The procedure involves reagent and plate preparation, primary incubation at 37°C, biotinylated detection antibody binding, HRP-Streptavidin (SABC) binding, TMB color development in the dark, and absorbance reading at 450 nm after addition of stop solution. The target corresponds to Uniprot P10827 and is also known by synonyms including THRA, c-erbA-alpha, c-erbA-1, Nuclear receptor subfamily 1 group A member 1, V-erbA-related protein 7, EAR-7, EAR7, ERBA1, NR1A1, THRA1, THRA2, AR7, ERBA, ERBA-related 7, and triiodothyronine receptor.