Human TGT (tRNA-Guanine Transglycosylase) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is configured as a double-antibody assay for the quantitative measurement of human tRNA-Guanine Transglycosylase (TGT; UniProt Q9BXR0), an enzyme that modifies transfer RNA molecules and has been linked to diseases such as cancer, genetic disorders, and neurodegenerative diseases. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid biological fluids.

Performance specifications include a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. The colorimetric detection method relies on a biotinylated detection antibody and HRP-Streptavidin conjugate with TMB substrate, with absorbance read at 450 nm. The kit is stored at 2–8 °C with a stated 12-month stability.

Capture antibody is pre-coated onto the plate, and target concentrations are calculated from a standard curve. The workflow proceeds through sequential incubation with sample, biotin-labelled antibody, and SABC working solution, followed by colour development, a stop reaction, and plate reading.