Human TGF-beta 2 PharmaGenie ELISA Kit (SBRS1142)

TGF-beta 2 is a key cytokine in immune regulation, fibrotic signalling, and embryonic development, and quantifying it in human samples supports research in oncology, wound healing, and pulmonary disease. This sandwich-format immunoassay from Assay Genie uses a monoclonal antibody pair pre-coated onto a 96-well microplate, with a standard curve range of 31.25 to 2000 pg/mL and a sensitivity below 10 pg/mL. The protocol requires 100 µL of cell culture supernatant, plasma, or serum per well, with a total incubation time of approximately 4.5 hours at room temperature. Reagents should be refrigerated between uses, and the kit includes a serial dilution standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and TMB substrate. Researchers studying epithelial-mesenchymal transition, glaucoma, or extracellular matrix deposition routinely use this assay to measure secreted TGF-beta 2 in conditioned media and biofluids.