Human TFPI2 ELISA Kit

This sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor 2 (TFPI2) in human samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. Built on a double-antibody sandwich format, the kit supports research into TFPI2, a protein implicated in regulating blood coagulation and angiogenesis, with dysregulation linked to cancer, cardiovascular disorders, and inflammatory conditions.

Performance specifications include a sensitivity of 46.875 pg/mL and an assay range of 78.125–5000 pg/mL. Recovery in serum, EDTA plasma, and heparin plasma averages 94%, 94%, and 97% respectively, with corresponding ranges of 88–100%, 88–100%, and 88–102%. Linearity at 1:2, 1:4, and 1:8 dilutions spans 85–104%, 85–105%, and 86–102% for serum, 82–99%, 82–100%, and 82–87% for EDTA plasma, and 82–97%, 85–100%, and 80–99% for heparin plasma.

Colorimetric detection is performed at 450 nm following TMB substrate development. The protocol involves a primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, and colour development in the dark for 10–20 minutes. The kit is stored at 2–8°C for 12 months and is classified under cardiovascular research applications. The associated UniProt entry is P48307.