Human TFF3/Trefoil Factor 3 ELISA Kit

Manufactured by Assay Genie, this ELISA kit enables quantitative measurement of human Trefoil Factor 3 (TFF3) using a sandwich ELISA double-antibody format with colorimetric detection. The assay is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and offers a sensitivity of 46.875 pg/mL across a working range of 78.125–5000 pg/mL. TFF3 is described as a key protein involved in maintaining mucosal integrity and promoting wound healing in the gastrointestinal tract, and its dysregulation has been associated with various gastrointestinal diseases and cancers, making this kit a valuable tool for related biomarker research. Synonyms listed for the target include TFF3, Trefoil factor 3, ITF and TFI.

Reagents are stored at 2–8°C for 12 months. The procedure involves equilibrating reagents and TMB substrate to room temperature, loading standards and samples on the pre-coated plate, incubating at 37°C for antigen binding, adding a biotin-labelled detection antibody followed by HRP-Streptavidin, developing with TMB substrate in the dark for 10–20 minutes, stopping the reaction and reading absorbance at 450 nm. Reported linearity across 1:2, 1:4 and 1:8 dilutions spans 81–99% for serum, 82–98% for EDTA plasma and 83–100% for heparin plasma, while average recovery is 94% for serum, 93% for EDTA plasma and 98% for heparin plasma.