Human TFF1 (Trefoil Factor 1) ELISA Kit

A Sandwich ELISA from Assay Genie enables sensitive quantification of human Trefoil Factor 1 (TFF1), a protein involved in maintaining mucosal integrity, promoting tissue repair, gastrointestinal health, wound healing, and cancer progression. The colorimetric, double-antibody assay is designed for the precise measurement of TFF1 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Key performance specifications include a sensitivity of 9.375 pg/mL and a working range of 15.625–1000 pg/mL. In validation testing, serum, EDTA plasma, and heparin plasma samples showed consistent linearity across dilution factors, with average spike-recovery values between 93% and 98%. All components should be stored at 2–8°C, where the kit remains stable for 12 months.

The workflow follows a straightforward sandwich ELISA protocol: equilibrate reagents and the pre-coated plate, incubate standards and samples for antigen binding, add biotin-labelled detection antibody followed by HRP-Streptavidin, develop colour with TMB substrate, and read absorbance at 450 nm after adding stop solution.