Human Tetraspanin 1 (TSPAN1) ELISA Kit

Tetraspanin 1 (TSPAN1) belongs to the transmembrane 4 superfamily, where it acts as a scaffolding protein that organizes cell membrane microdomains involved in adhesion, migration, and intracellular signaling. The Assay Genie human TSPAN1 ELISA kit enables researchers to quantify this protein in human specimens, supporting oncology biomarker investigations, tumor biology research, and mechanistic work on tetraspanin-enriched microdomains.

TSPAN1 has attracted attention as a candidate biomarker in hepatocellular carcinoma, gastric cancer, and colorectal carcinoma, where altered expression has been observed in tumor tissues compared with matched normal controls. Investigators use the kit to profile patient-derived cohorts, validate knockdown or overexpression experiments in cell culture models, and explore functional roles in cell adhesion, proliferation, and migration pathways. Published literature also points to roles in immune cell behavior and pathogen entry into host cells, broadening the assay's relevance across multiple research programs.

Each kit ships with pre-coated microplate wells, biotinylated detection antibody, standards, and assay buffers sufficient for 96 determinations. Compatible input materials include serum-based preparations, plasma-derived samples, and tissue homogenates prepared from human donors. Researchers should store reagents refrigerated, protect components from light, and aliquot standards to limit freeze-thaw cycles across multiple runs.

The assay is intended for research use only, and laboratories can consult the lot-specific certificate of analysis for current performance characteristics such as expected standard curve behavior and dynamic range. Detailed protocols guide users through incubation times, washing steps, and data interpretation for downstream in vitro studies, with typical read time falling within a few hours from sample addition to final quantification.