Human TERT/Telomerase Reverse Transcriptase ELISA Kit

Designed for the quantitative measurement of Telomerase Reverse Transcriptase (TERT) in human samples, this Sandwich ELISA Kit from Assay Genie uses a colorimetric, double-antibody format intended for a variety of research applications. TERT is a vital enzyme responsible for maintaining telomere length and plays a key role in cellular aging and cancer development, making it a useful biomarker for understanding disease mechanisms and identifying therapeutic targets.

The kit is reactive with human samples and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported recovery values are 87–104% (average 94%) for serum, 86–104% (average 94%) for EDTA plasma, and 85–105% (average 96%) for heparin plasma (n=5 each), with matching performance across dilutions of 1:2, 1:4 and 1:8 for each matrix. Kit components are stored at 2–8°C for 12 months.

The assay workflow begins with equilibration of reagents and TMB substrate to room temperature, followed by loading of standards and samples onto the pre-coated plate and incubation at 37°C for 90 minutes. A biotin-labelled detection antibody is added and incubated for 60 minutes, then HRP-Streptavidin for 30 minutes. TMB substrate is added and incubated in the dark for 10–20 minutes before stop solution is applied and absorbance is read at 450 nm.