Human Teratocarcinoma Derived Growth Factor 1 (TDGF1) ELISA Kit

Quantitative measurement of human Teratocarcinoma-Derived Growth Factor 1 (TDGF1, also known as Cripto-1) in research samples is enabled by this ELISA Kit from Assay Genie, intended for research use only in in vitro workflows. TDGF1 is a GPI-anchored signaling protein that serves as an essential co-receptor in the Nodal branch of the TGF-β pathway, where it is required for gastrulation, left-right asymmetry, and anterior-posterior axis specification during embryonic development.

Outside its developmental role, TDGF1 is frequently re-expressed in carcinomas of the breast, lung, colon, stomach, and pancreas. Elevated tissue and circulating levels have been linked to higher proliferative index, increased invasive and metastatic capacity, and reduced patient survival in multiple tumor types. This positions the protein as a research target for oncology biomarker discovery, mechanistic studies of epithelial-to-mesenchymal transition, and screening of candidate therapeutics aimed at the Nodal signaling axis.

The kit is supplied in microplate format and provides 96 assays per box with a colorimetric read-out, validated for reactivity with human samples. It accommodates a variety of specimen inputs, including tissue homogenates and cell lysate preparations, alongside other biological matrices encountered in cell biology and translational research workflows.

Typical research applications include quantifying TDGF1 release into conditioned media from pluripotent stem cell cultures, profiling tumor-derived cell lines, and tracking protein abundance in patient-derived tissue specimens to investigate developmental signaling, stem cell maintenance, and cancer biology.