Human Tenascin-N (TNN) ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit is specifically designed for the precise measurement of human Tenascin-N (TNN) levels in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. Using a colorimetric detection method, the assay targets TNN — a key extracellular matrix glycoprotein involved in cell adhesion, migration and tissue morphogenesis, and implicated in wound healing, tissue remodelling and cancer metastasis.

The kit delivers a detection range of 0.312-20ng/mL with a sensitivity of 0.1ng/mL. Precision performance is reported as an intra-assay CV of 4.3% and an inter-assay CV of 8.0%. Dilutional linearity for serum falls within 83-106%, for EDTA plasma within 94-114% and for heparin plasma within 86-117%, across 1:2 to 1:16 dilutions. Recovery averages 94% for serum (range 88-100%) and 96% for plasma (range 90-102%). The assay specifically recognises natural and recombinant human Tenascin-N (UniProt Q9UQP3), a homohexameric secreted extracellular matrix protein with a molecular weight of 144,034 Da.

Intended for research use only, this Signal Transduction-focused kit is suitable for biomarker discovery and investigations into the role of tenascin-N in health and disease, including its activity as a ligand for integrin complexes such as ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1, and its contributions to neurite outgrowth, cell migration and tumour angiogenesis.