Human TdT/DNA nucleotidylexotransferase ELISA Kit

Designed for research applications, this Sandwich ELISA from Assay Genie enables quantitative measurement of TdT/DNA nucleotidylexotransferase in human samples, supporting work in epigenetics and nuclear signalling. The assay uses a colorimetric detection method and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reported average recovery values are 94% for serum (n=5), 95% for EDTA plasma (n=5), and 93% for heparin plasma (n=5). Linearity across 1:2, 1:4 and 1:8 dilutions is given as 85-104%, 83-98% and 82-99% for serum; 91-104%, 87-99% and 82-99% for EDTA plasma; and 85-102%, 84-100% and 82-101% for heparin plasma (each n=5). The kit is stored at 2-8°C for 12 months.

The associated UniProt accession is P04053, with listed synonyms including DNTT, DNA nucleotidylexotransferase, TDT, Terminal addition enzyme, Terminal deoxynucleotidyltransferase and Terminal transferase. According to the supplied protocol, the workflow involves antigen incubation at 37°C, biotin-labeled detection antibody binding, HRP-Streptavidin (SABC) interaction, TMB colour development in the dark, and absorbance measurement at 450 nm.