Human TDG ELISA Kit (HUFI06159)

The TDG (thymine DNA glycosylase) enzyme initiates base excision repair by excising mispaired thymine and uracil bases from G:T and G:U wobble mismatches, and it processes 5-formylcytosine and 5-carboxylcytosine intermediates generated during active DNA demethylation. Quantifying TDG protein levels in research samples supports investigations into epigenetic regulation, DNA repair capacity, and the molecular pathology of cancers and autoimmune conditions.

This Assay Genie ELISA kit is supplied as a microplate containing capture antibody, detection antibody, standards, and substrate reagents sufficient for 96 individual determinations. The two-antibody format produces a chromogenic read-out proportional to TDG concentration when the plate is analyzed on a standard microplate reader. The kit is intended for in vitro research use only and is configured for measurement of human thymine DNA glycosylase, with the antibody pair raised against the human TDG sequence.

Typical research applications include biomarker quantification in tumor lysates and tissue homogenates, characterization of base excision repair activity in cell culture models, and mechanistic work on TET-mediated demethylation pathways. The plate-based workflow is compatible with standard laboratory equipment and integrates into existing pipelines for DNA repair, epigenetics, and molecular oncology research.