Human TCTN2 ELISA Kit (HUFI06157)

TCTN2 (Tectonic Family Member 2) is a ciliary transmembrane protein that forms part of the tectonic complex regulating ciliogenesis and Hedgehog signal transduction. Loss-of-function variants in TCTN2 are linked to Joubert syndrome 24 and Meckel syndrome type 8, making quantitative measurement valuable for ciliopathy and developmental biology research. The Human TCTN2 ELISA Kit from Assay Genie provides an immunoassay format for measuring human TCTN2 in serum or plasma specimens, supporting studies in cell signaling, neurodevelopment, and disease modeling.

The kit is configured as a 96-reaction microplate-based immunoassay and includes pre-coated plate strips, reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, TMB substrate, and stop solution. The procedure involves sequential incubation of the sample, detection reagent, and color development substrate, with absorbance read on a standard plate reader. Reconstituted reagents should be refrigerated and used within the kit's stated shelf life, with the sealed kit stored cold and protected from light to preserve reagent activity.

Intended for research use only, this kit is not approved for diagnostic or therapeutic applications. Each unit provides reagents sufficient for 96 individual reactions in a single-use configuration. The assay is validated for human-derived specimens. Researchers should refer to the supplied manual for lot-specific performance parameters, recommended dilution ranges, and standard curve characteristics.