Human TCF21/Transcription factor 21 ELISA Kit

Designed by Assay Genie for research applications, this ELISA kit enables the quantitative measurement of human TCF21 (Transcription factor 21). It is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid sample types. The kit employs a sandwich ELISA methodology and is intended for investigations within the epigenetics and nuclear signalling research area.

Key performance characteristics include a sensitivity of 0.469 ng/mL and a working range of 0.781–50 ng/mL. Reagents should be stored at 2–8 °C, where they remain stable for up to 12 months. The assay procedure involves equilibrating reagents, incubating standards and samples on a pre-coated plate, binding with a biotin-labelled detection antibody, adding HRP-streptavidin conjugate, developing colour with TMB substrate, and reading absorbance at 450 nm after adding stop solution.

TCF21 is a transcription factor that regulates gene expression and plays a critical role in cell differentiation and development. Dysregulation of TCF21 has been implicated in cardiovascular disorders, obesity, and cancer, making it a biomarker of interest for studying disease pathogenesis and exploring therapeutic interventions. The kit is associated with the UniProt entry O43680 and is also known by synonyms including POD1, Epicardin, Capsulin, and bHLHa23.