Human TANK Binding Kinase 1 (TBK1) ELISA Kit

Quantitative measurement of TANK-binding kinase 1 (TBK1) from Assay Genie is intended for research on this serine/threonine kinase in human tissue homogenates and cell lysates using a colorimetric microplate readout. TBK1 sits at the crossroads of innate immune signaling, where it phosphorylates IRF3 and IRF7 to drive type I interferon production downstream of pattern-recognition receptors such as cGAS-STING, TLR3, and RIG-I, and it also contributes to NF-kB pathway activation and selective autophagy. These roles make the kit broadly relevant to studies of antiviral host defense, inflammatory cytokine cascades, tumor-intrinsic innate immunity, and the cellular response to cytosolic nucleic acids.

Researchers in neurodegeneration also use TBK1 quantification because loss-of-function mutations in the TBK1 gene are a recognized cause of familial amyotrophic lateral sclerosis and frontotemporal dementia, and altered kinase activity has been reported in cellular and animal models of these conditions. The kit is supplied as a pack of 96 assays on a microplate, calibrated for relative expression studies, treatment-time-course experiments, and side-by-side comparison of TBK1 levels between experimental and control cohorts. Each run follows an in vitro protocol with colorimetric signal acquisition on a standard plate reader, supporting workflows from basic cell biology through translational immunology.

Compatibility with cell lysates and tissue homogenates, together with documented human reactivity, makes the assay a practical choice for laboratories investigating kinase expression or pathway activation across diverse experimental systems.