Human TAF3 ELISA Kit (HUFI06203)

Quantitative ELISA kit from Assay Genie targeting TATA-box binding protein-associated factor 3 (TAF3), a subunit of the TFIID transcription complex that recognizes H3K4me3 histone marks and supports RNA polymerase II-mediated transcription initiation. The kit enables measurement of human TAF3 in research samples using a colorimetric readout, supporting studies of transcriptional regulation, chromatin biology, stem cell maintenance, and oncogenic signaling pathways where TAF3 expression or function is altered. Each kit provides reagents for 96 assays in a microplate format, with pre-coated wells, biotinylated detection antibody, standards, and substrate components supplied ready for standard curve and sample quantification. The protocol follows standard ELISA workflow: capture antibody on the plate, incubation with standards or samples, sequential addition of detection reagent and chromogenic substrate, then absorbance reading on a microplate reader. Unopened components should be kept refrigerated and protected from light to preserve activity through the stated shelf life; allow reagents to equilibrate to room temperature before each run. Researchers investigating TFIID complex function, epigenetic reader proteins, or TAF3 dysregulation in tumor samples will find this assay a complementary tool alongside Western blot and qPCR approaches for characterizing gene expression control.