Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC ELISA Sampler Kit

The Assay Genie Human T Cell Co-inhibitory and Co-stimulatory Receptor IHC ELISA Sampler Kit bundles seven separate 48-well microplates, each pre-configured to profile a distinct immune checkpoint target, enabling simultaneous interrogation of suppressive pathways such as PD-1, CTLA-4, TIM-3, LAG-3 and TIGIT alongside activating receptors including CD28, ICOS, 4-1BB, OX40, CD27 and GITR from the same donor set. Each well plate is coated with human-specific capture antibodies binding a single receptor, supporting both immunohistochemistry staining of fixed tissue sections and quantitative ELISA readout of circulating biomarkers across biofluid matrices. The chromogenic absorbance chemistry produces a measurable signal proportional to receptor abundance, and the modular plate design lets laboratories run individual targets independently or in parallel without committing to a full single-analyte kit. Researchers studying tumor microenvironment immunology, autoimmune biomarker discovery, or checkpoint inhibitor response prediction will find the multi-target panel efficient for screening cohorts where sample volume is limiting, since each analyte requires a 50 uL sample per well. Unopened plates should be refrigerated until first use, and reconstituted components handled according to the package insert to preserve antibody binding activity across multiple freeze-thaw cycles. The 7 x 48 assay format totals 336 wells, providing flexibility for both small pilot studies and larger translational research programs investigating human T cell exhaustion and activation dynamics.