Human T-box transcription factor TBX3/TBX3 ELISA Kit

A high-quality sandwich ELISA, double antibody assay from Assay Genie is designed for the quantitative measurement of human T-box transcription factor TBX3 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric, using a biotinylated detection antibody, HRP-streptavidin conjugate, and TMB substrate with absorbance read at 450 nm.

Sensitivity is 0.188 ng/mL and the working range is 0.313–20 ng/mL. Reported linearity across serum, EDTA plasma, and heparin plasma dilutions falls between 86–105%, with average recovery of 96–99% across the same matrices. The kit should be stored at 2–8°C for 12 months.

TBX3 is a transcription factor involved in embryonic development, cell proliferation, and differentiation, and its dysregulation has been linked to cancer, developmental disorders, and cardiovascular disease. The associated Uniprot reference is O15119, and the research area is epigenetics and nuclear signaling. Kit components include a pre-coated dismountable microplate, lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate (SABC, 100×), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, 25× wash buffer, plate sealers, and a technical manual.