Human Syntaxin 4 ELISA Kit (HUFI08404)

Syntaxin 4 (STX4) is a ~34 kDa plasma-membrane t-SNARE protein that drives vesicle fusion and insulin-stimulated GLUT4 glucose transporter translocation, making it a relevant target in metabolic and membrane-trafficking research. This Assay Genie reagent provides quantitative measurement of STX4 in human specimens through an absorbance-based immunoassay run on a microplate, with reagents supplied for 96 Assays per kit.

Components include pre-coated wells, recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, and wash buffer, with a complete protocol supplied for straightforward setup. The assay accepts serum or plasma specimens as well as cell and tissue lysate research samples, supporting studies of insulin signaling, pancreatic beta-cell exocytosis, and diabetes-related investigation.

Researchers also apply the kit to characterize STX4 expression in adipocyte and myocyte model systems. Reagents should be stored refrigerated and brought to room temperature before each run; aliquot working solutions to limit freeze-thaw cycles of the standards. The kit is labeled for research use only and is supplied in a microplate format with lyophilized standard calibrators.