Human Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit

Quantitative measurement of Suppressors of Cytokine Signaling 2 (SOCS2) supports research into JAK-STAT pathway regulation, growth hormone signaling, and cytokine-mediated immune responses. This Assay Genie ELISA Kit detects human SOCS2 in tissue homogenates using a microplate-based chromogenic readout, with each kit providing reagents sufficient for a full plate of determinations.

SOCS2 functions as a negative feedback regulator within cytokine receptor signaling, primarily restraining STAT5 activation downstream of growth hormone and prolactin receptors. Altered SOCS2 expression has been documented in hepatocellular carcinoma, breast cancer prognosis studies, and immune cell differentiation research, making quantitative assessment useful for translational projects in oncology and immunology.

The package includes a pre-coated plate, lyophilized reference standard, biotinylated detection reagent, enzyme conjugate, substrate solution, wash buffer, and stop reagent. Following the supplied protocol, investigators add diluted samples and standards to the plate, perform sequential incubations, then develop color proportional to bound SOCS2. A standard curve generated from serial dilutions allows interpolation of unknown concentrations. Reconstituted reagents should be refrigerated and used within the working session according to the product insert.

This research-use-only assay supports academic and pharmaceutical laboratories examining SOCS2 as a pathway biomarker. The kit is intended for laboratory use by trained personnel familiar with ELISA methodology and standard curve analysis.