Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA Kit

Designed for quantitative measurement of human Cu/Zn superoxide dismutase [Cu-Zn] (SOD1), this Assay Genie sandwich-format colorimetric ELISA is for use with serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. Specificity covers natural and recombinant human Superoxide dismutase [Cu-Zn].

The detection range is 0.156-10 ng/mL with a sensitivity of 0.078 ng/mL. Intra-assay CV is 5.1% and inter-assay CV is 8.6%. Across 1:2 to 1:16 dilutions, serum (N=5) linearity ranges from 82-82% to 104-113%; EDTA plasma (N=5) from 80-90% to 93-103%; heparin plasma (N=5) from 107-119% to 104-114%. Recovery averages 88% (range 82-94%) in serum and 90% (range 84-96%) in plasma.

Cu/Zn SOD1 (UniProt P00441) is a homodimer that destroys radicals normally produced within cells and which are toxic to biological systems. Dysregulation has been implicated in neurodegenerative disorders, ALS, and cardiovascular diseases. The protein localises predominantly to the cytoplasm, with additional subcellular distribution in mitochondria and nucleus. Research area: neurosciences. For research use only.